Cytarabin was nanoniosomated using non ionic surfactant through ether injection method. The percent entrapment efficiency was found to be 98%. This was calculated based on passing niosomated cytarabine onto column of Sephadex G-50 and the obtained fractions were pooled and ruptured in order to determine per cent of unentrapped cytarabine. The released data were fitted in to different popular kinetic models. The released of cytarabine from the niosome was followed by first order kinetic and Hixson Crowell model. The size of nano niosomes containing cytarabine was determined to be 347nm with the zeta potential of 12.7 mV. ThusIC50 values for the pure drug and nano niosomated formulations indicate the higher effectiveness of the cytarabinenoisomal formulation.