Acoustical studies have been conducted on the binary mixtures of Amitriptyline and Benzene at 2.9979 MHz and at different temperatures from 299K to 328 K using Ultrasonic interferometer. The experimentally determined ultrasonic velocity and density are used to calculate the various thermo-acoustical parameters like adiabatic compressibility (β), intermolecular free length (Lf), specific acoustic impedance (Z), molar volume (Vm), available volume Va(s), Rao’s number (Ra) and Wada’s number (W). From these various parameters the molecular interactions present in the binary mixture are studied with respect to change in temperature. It is observed that, there are weak molecular interactions present between the components of the mixture and these interactions are found to be decreasing with increase in temperature.