The objective of this work is to propose a new process to make biodiesel from chicken waste by the transesterification process. The experimental part presents a method to recover fat wastes from the chicken slaughterhouse process and extract the anhydrous fat from it. The final product obtained by transesterification was biodiesel fuel, which can be used as an alternative fuel. The glycerin obtained as byproduct must be further processed to compete in quality with synthetic glycerol. Various tests were conducted to maximize the transesterification reaction. Thus with one kilogram of chicken wastes (heads, stomachs ...) we recovered 300g of anhydrous fats. The transesterification in the presence of 1.1% KOH, with a catalyzed reaction at 60°C, gives a yield of 98% in biodiesel. The comparison of our results with literature showed that the manufacture of biodiesel from chicken waste gave similar results as from pig and beef fats.