Plant based products are healthier, safer and much more reliable than the synthetic products. According to WHO, approximately 80% of the world’s inhabitants rely on traditional medicine for their primary health care. It was reported that during the past 20 years, at least one novel compound from higher plants has been marketed every 2.5 years. This proves the importance of ethno pharmacy in today’s research. The current study explains about the antibacterial activity of the petroleum ether and ethyl acetate plant extract of three sided Cissus quadrangularis. The antibacterial potential of the crude plant extract obtained by soxhlet extraction was determined by disc diffusion and well diffusion assays using both gram positive organisms (Bacillus cereus and Staphylococcus aureus) and gram negative organisms (Escherichia coli and Salmonella typhi). The results showed that the ethyl acetate plant extract was highly effective against all the organisms compared to petroleum ether extract. Among the bacterial strains, both the extracts showed maximum zone of inhibition against Staphylococcus aureus. Phytochemical analysis proved the presence of constituents such as flavanoids, carbohydrates, tannins, phenols and saponins. Thus, this study proved that the extracts of three sided Cissus quadrangularis possess valuable phytochemicals and it could act as an effective antibacterial agent.