The aim of this study was to develop and evaluate pulsincap of Aceclofenac and serratiopeptidase using different amount of HPMC K15 M and di calcium phosphate as plugging materials. This formulation is planned to use for better therapy of inflammatory pain in patients with various inflammatory disorders with a predetermined lag time. Granule equivalent to 100 mg of Aceclofenac and 10 mg serratiopeptidase were accurately weighted and added into the treated formaldehyde bodies by manual hand filling method. The bodies were then plugged with different polymers like hydroxylpropylmethylcellulose K 15 M and dicalcium phosphate. Plugs were punched by rotary compression machine called as tablet plugs. Then capsule body and cap is joined and sealed with a small amount of ethanolic ethyl cellulose solution. Plug composition was optimized by using 2 factor 3 level factorial designs. Plug Amount and % of Polymer in plug were taken as independent factor in the present study. Lag time of rupture of PRTs as dependent variable in this study. FT IR study is used for concluding compatibility of drugs with excipients. The prepared formulation was tested for Rupture Test, In vitro dissolution study and in vivo study. Results of all in vitro study and in vivo study would also justify pulsatile release of drugs from dosage form.