To develop chromatographic fingerprint of total glycosides of Bazhen decoction and simultaneous determination of three components，including paeoniflorin , ginsenoside Rg1 and ginsenoside Rb1. Total glycosides of Bazhen decoction were separated and purified using a macroporous adsorption resins method, HPLC-DAD method was applied for fingerprint of total glycosides and simultaneous determination. The mobile phase, consisting of acetonitrile- phosphoric acid solution(0.05%),was programmed for a gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was monitored at 203 nm, and the column temperature was maintained at 40 ℃. There were 15 common peaks in the chromatographic fingerprint，among these peaks, peak 1,9,13 were identified as paeoniflorin, ginsenoside Rg1, ginsenoside Rb1, respectively. The contents of 3 components were detected in the same chromatographic conditions. This study indicated that the combination of chromatographic fingerprint and quantitative analysis can be readily utilized as comprehensive evaluation of total glycosides of Bazhen decoction.