The present work has been designed to study the effect of hot aqueous leaf extract of Anthocephalus cadamba (Roxb.) Miq, Rubiaceae, on haematological and biochemical parameters of wistar albino rats. Different group of rats were administered orally with doses like 125mg/kg, 250mg/kg and 500mg/kg body weight of leaf extract. Treatment with different doses of leaf extract significantly (p <.05) increases the level of haemoglobin (Hb), packed cell volume (PCV), red blood cells (RBC) and white blood cells. The levels of albumin, urea, creatinine and bilirubin in wistar albino rats fed with different doses of leaf extract were not significantly different form control. Various doses of aqueous leaf extract significantly (p<.05 and .01) reduced the levels of glucose, total cholesterol, alanine amino transferase (ALT) and aspartate amino transferase (AST). Glucose, total cholesterol, ALT and AST decrease in accordance with the dose given.