An efficient vaccine which would stimulate both humoral and cell mediated immune response is the ideal solution for HIV/AIDS problem. CD4+ T cells play a significant role in induction and maintenance of CD8+ T cell and antibody-producing B cell responses their by aid a tremendous role HIV disease control. Our study based on immunoinformatics approach focus on the prediction of HLA-DRB1*10 allele specific epitopes capable of triggering immunogenic activity. IEDB method was adopted to identify Gag epitopes vaccine candidates MYSPISILDIKQGPK-P1, RMYSPISILDIKQGP-P2, YSPISILDIKQGPKE-P3, VPVGEIY KRWIILGL-P4, PVGEIYKRWIILGLN-P5 restricted to HLA-DRB1*10 allele would aid significant CD4+ T cell immune response against HIV infection and population coverage among south Indian population were assessed. Three dimensional structures of epitopes P1-P5 modeled using I Tasser, and their insights of binding affinity towards HLA binding groove analyzed by ClusPro based docking studies resulted with conventional hydrogen bonding. Thus the interaction between the screened P1-P5 epitopes and DRB1*10 alleles exhibiting stability and would aid immune response. Current data would provide the insights for design and development of novel GAG based vaccine candidate against HIV infection