Rapid and accurate reverse phase high performance liquid chromatography method is described for determination of ofloxacin from the bulk drug and pharmaceutical dosage form. It was observed that water symmetry C8 (15 x 4.6 mm i.d.) with 5 μ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of buffer and acetonitrile (85:15 % v/v). The buffer was mixtures of 1 % tri-ethyl amine adjusted the pH 2.6 with ortho-phosphoric acid. The detection was carried out at wavelength 294 nm. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution with the linear range 10-30 μg/ml. All the peaks of degradation products were resolved from the active pharmaceutical ingredient with significantly differ retention time. As the method could effectively separate the drug from its degraded products, hence above method can be also used for study of stability indicating parameters. The method has been successfully used to assay of pharmaceutical dosage form i.e. tablets with good recoveries.