Many transitional metal ion complexes have been synthesized for their analytical and commercial applications. The complexes of the type [ ML2.2H2O] Cl2 {where M= Mn(II), Ni(II), Co(II), Cu(II) and Zn(II) and L=4-{(E)-[(2- chlorophenyl)imino]methyl}benzene-1,2-diamine}have been prepared and characterized on the basis of Elemental analysis,IR, UV-Visible, ESR, Mass, Magnetic susceptibility, Microbial activities, Conductivity measurements, Thermal analysis and X-ray powder diffraction. These complexes are 1:2 ratios, the magnetic data coupled with electronic spectra and ESR suggests that complexes are square planar geometry. IR of the ligand and complexes agree with the co-ordination to be central metal atom through both the nitrogen atoms. The electronic spectra bonding parameter ( b½ ) and nephelauxetic ratio (β) have been also evaluated. Conductance measurements suggest that complexes are 1:1 electrolytic in nature. The X-ray powder diffraction studies indicate that, the unit cell is monoclinic and space group of complexes is C2/m or Pc/m. Microbial activity of ligand and complexes show more activity towards Gram positive and Gram negative.