The phosphate solubilizing microorganisms were isolated from the groundnut rhizosphere soil. The isolated colony were confirmed as Bacillus subtilis and Bacillus cereus. The effect of different pH, temperature, carbon sources, nitrogen sources, potash sources on the phosphate solubilization was optimized. Maximum solubilization was recorded at temperature range 400C.Screening of phosphate solubilizing bacteria was done in Bacillus subtilis, Bacillus cereus in both solid & liquid medium..Bacillus subtilis is the most efficient for phosphate solubilizing activity, at pH 7, Carbon sources [0.35+0.02]Nitrogen sources sources[0.28+0.03]Potash sources[0.12+0.09] strains on the basis of their phosphate solubilizing activity. Further research should be continued with efficient phosphate solubilizing isolates and their antimicrobial activity could be exploited as biofertilizer as well as bio pesticide. Chemical fertilizers are predominantly used to enhance crop yield but continuous use causes various draw back like sterility of soil. The use of biofertilisers avoids side effects and retains the fertility of the soil and can be well exploited as phosphatic biofertilizers Screening of phosphate solubilizing bacteria in both solid & liquid medium. Quantitative analysis of organic acid produced by phosphate solubilizing Bacteria.