A simple, specific, and accurate reverse phase liquid chromatographic method was developed for the simultaneous estimation of Levodopa (LD) and Carbidopa (CD) in bulk and pharmaceutical dosage forms. A Phenomenex C18- RP Aqueous reverse phase column (250 x 4.6mm, 5 μm) with mobile phase containing 0.05%(v/v) o-phosphoric acid: acetonitrile (96:4%v/v) was used at 1mL/min flow rate in isocratic mode and the eluents were monitored at 220 nm. The retention times of LD and CD were 4.2 and 7.4 min respectively and showed a good linearity in the concentration range of 20-100 μg/mL for LD and 10-50μg/mL for CD with a correlation coefficient (R) of 0.9999 and 0.9998. The percentage assays for controlled matrix tablets (SYNDOPA) were found to be 101.98 and 100.47 respectively for LD and CD. The proposed method was validated as per ICH guidelines and successfully applied for the simultaneous estimation of LD and CD in tablet formulations and dissolution sample analysis.