An accurate, simple, precise, specific and robust High Performance Thin Layer Chromatography (HPTLC)method was developed and validated for simultaneous estimation of cefixime trihydrate and ornidazole. The two compounds were separated on TLC plate Silica gel 60 f254 using dual run method of mobile phase initially with pure methanol and then with pure ethyl acetate. After development, the plate was evaluated by densitometry, the HPTLC Scanner 3 of CAMAG. The tow compounds separated in a good resolution and Rf values found to be 0.23±0.003 for Cefixime trihydrate and 0.67 ±0.003 for Ornidazole.The method has been validated as per ICH guideline (Q2 R1) for the parameters of Linearity and Range, Accuracy, Precision, Specificity, Limit of Detection (LOD), and Limit of Quantification (LOQ). The method also has been successfully applied for analyzing of two brands of tablet marketed dosage form of combined drugs (MAHACEF OZ& ZIFI-OZ).