To analyse the two bioactive components Gallic acid and Quercetin from the methanolic extract of aerial part of Anisomeles malabarica R.Br. Ex Sims. Chromatography Separation was carried out on aluminium plate precoated with silica gel 60F254 with toluene: ethyl acetate: formic acid (5.0:2.5:0.5, v/v/v) as solvent system. Densitometry scanning was performed under reflectance- absorbance mode at 366 nm to quantify the spots. The Rf values of Gallic acid and Quercetin were found to be 0.24 and 0.40 respectively. Linearity of r2 = 0.999 and 0.998, LOD 0.6 and 0.7 μg/band, LOQ 0.7 and 0.8 μg/band and recovery of 97.62% and 97.55% respectively were satisfactory for Gallic acid and Quercetin. The HPTLC method was validated as per the ICH guideline. The developed simple, precise, specific, sensitive and accurate method can be used for routine quality control of raw material of Anisomeles malabarica R.Br. Ex Sims as well as formulation containing any of these bioactive compounds.