Otitis media, an inflammation of the middle ear, is a common illness in childhood and also occurs in adults. This study aimed to investigate the correlation between chronic otitis media (COM) and acute otitis media (AOM) and the levels of serum Interleukin-6 (IL-6) and C- reactive protein (CRP) in patients contact clinics in Damascus hospital in period 2013–2014.This study involved 77 patients with otitis Media (OM), (COM: 45 vs AOM: 32) and 22 healthy people as a control group (age: 2-70 year). The results showed that both IL-6 and CRP were significantly increased (p<0.05) in serum of COM patients (Pseudomonas aeruginosa: 5.12±0.664 pg/ml, 6.72±0.624 mg/l) and in serum of AOM patients(Streptococcus pneumonia: 48.60±3.35pg/ml, 63.88±3.102mg/l)as compared to healthy controls. The serum IL-6 and CRP levels from bacterial COM patients showed a little elevations. Contrarily, the serumIL-6 and CRP levels from bacterial AOM patients showed increased elevations especially in streptococcal AOM patients. Therefore, serum IL-6 and CRP would be a diagnostic biomarkers for bacterial otitis media.