A study on the seroprevalence of influenza virus in broilers was carried out. A total of 570 broilers were randomly collected from 52 poultry farms of Quetta and its surroundings to determine the seroprevalence and antibody titre of avian influenza virus in the sera of broilers. The seroprevalence of avian influenza virus in the sera of broilers was demonstrated by Enzyme Linked Immunosorbent Assay (ELISA). The sero-positive prevalence of avian influenza virus was recorded 14.03% (n=80) in broilers. All the positive sera of boilers determined by ELISA were further tested by using H5, H7, and H9 specific strains antigen through Haemagglutination Inhibition and Haemagglutination tests, only H9 was recognized from the sera of broilers. During present study the antibody titre of H9strain of avian influenza virus in the sera of broilers was investigated. The antibody titre of H9 strain of avian influenza virus was measured in the sera of broilers ranged from 1:8 to 1:164. It is concluded from the present investigation that avian influenza virus H9 strain is prevailing in broilers and around Quetta.