Cumin essential oil was extracted from cumin seeds with low boiling-point organic solvents by Soxhlet extraction method. In this paper, the extraction technology conditions were explained in detail. The cumin essential oil was separated and identified by Column Chromatography and GC-MS. Results showed that optimum petroleum ether extraction conditions for essential oil were as follows: extraction temperature is 33℃, extract time is 10h and the Cumin essence oil of maximum yield is about 82 mg/g. Through the component separation and analysis of the main chemical components, the biochemically active bases of essential oil which can inhibit bacterium in foodstuff antisepsis were ascertained. The method was Soxhlet extraction and gas chromatography-mass spectrometry, which can effectively determine the bacteriostatic components of cumin essential oil.