The chemical composition and antifungal activities of the volatile fractions from leaves, roots and flowers of Lupinus pilosus Murr. harvested in winter and spring were studied. The yields of volatiles ranged from 0.01 to 0.04%. Those in spring were higher than in winter. The GC and GC-MS analysis permitted us to identify 83 compounds. The harvest season and the studied organ affected qualitatively and quantitatively the percentage of the different constituents. Spathulenol (1.0 to 8.7%), globulol (2.3 to 8.7%), α -bisabolol (7.0 to 8.8%) and nonanal (2.8 to 16.3%) were the major compounds of the studied volatile fractions. Non- terpene derivatives (14.3 to 72.5%) and oxygenated sesquiterpenes (6.4 to 33.4%) were the most represented chemical classes. The antifungal properties depended on the studied organ and the harvest season. The fraction obtained from roots harvested in winter showed moderate antifungal activity against C. glabrata and C. krusei (ΦIZ= 10 mm).