The main objective of this study was to investigate the pharmacological activity of M. alysson L. extract collected in Egypt using experimental animal models. M. alysson L. used in this study was collected from Northern Sinai in Egypt and was air-dried in the shade and ground to a fine powder (3 Kg) which extracted by maceration with 90 % methanol, filtered, and evaporated using rotary evaporator till dryness. In this study, Diabetes mellitus was induced in male albino rats by intraperitoneal injection of Alloxan. The antipyretic activity was evaluated using pyrexia induced by yeast injection. Acetic acid writhing test was used to evaluate the analgesic activity, and rat paw edema method was used to evaluate the acute anti-inflammatory activity. The antiulcer activity of the extract was evaluated using indomethacin-induced ulcer method. Finally, metabolic cages were used to study the diuretic activity of the extract. Results showed that M. alysson L. extract in a dose of 100 mg/kg exhibited improvement in the antioxidant activity. In addition, the extract caused a significant reduction in elevated temperature after two hours as well as reduced number of acetic acid writhing movements, indicating a significant analgesic effect. Furthermore, the extract significantly reduced the carrageenan-induced paw edema through an anti-inflammatory effect. In terms of gastro protection, M. alysson L. reduced the number of ulcers induced by indomethacin and possess diuretic properties. In conclusion, M. alysson L. extract collected in Egypt showed antioxidant, antipyretic, analgesic, antiinflammatory, antiulcer as well as diuretic activity.