This study was aimed to evaluate the antimicrobial activities of Barlaria buxifolia Linn. has been studied by agar diffusion method. The carbon tetrachloride, ethyl acetate, ethanol and methanol extracts were prepared using soxhlet apparatus. The result of antimicrobial assay revealed that the extracts showed significant inhibitory activity against all the tested pathogens compared with standard and significant activity was observed with ethanolic and methanolic extract. Ethanol and methanol extracts revealed the presence of alkaloids, flavonoids, phenolic compounds, saponins and tannins. Thus, the results depict that stem extracts of B.buxifolia could be used as potential source of antibacterial and antifungal agents in the treatment of infectious diseases.