The present study was carried out on 83 isolates from marine (Mediterranean and Red Seas) and soil (seawedge from El-Fayoum and El-kanater) habitats. After three days of incubation the crude exopolysaccharides were separated and examined by DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical. Seven isolates from soil and 22 isolates from marine habitats showed antioxidant activity. The highest nine isolate showed antioxidant activities were identified as follow M7 (Bacillus circulans) (98.10%) M8 (Bacillus licheniformis) (97.34%), S1 (Bacillus alvei) (95.83%), M3 (Bacillus insolitus) (85.01%), S22 (Bacillus polymyxa) (84.31%), M9 (Bacillus marinus) (83.26 %), M6 (Bacillus anthracis) (81.50), M5 (Staphylococcus sp.) (80.42%) and M4 (Bacillus brevis) (76.90%) isolates.