The HPLC method has been successfully applied to detect some indole alkaloids in the three cultivars of Catharanthus roseus plants to facilitate further study of the metabolic pathway and its regulation in Catharanthus roseus plants; the Albus cultivar is the richest in content then Ocellatus and the poorest is Roseu. While on intensity level of valuable components Ocellatus cultivar has more advantages than Albus and Roseus respectively. Albus cultivar had the highest content of vincristine (1.5 fold) and catharanthine (1.6 fold); while Ocellatus cultivar had the highest content of vinblastine (1.7 fold), vindoline (6.6 folds) and tryptophan (1.7 fold); Roseus cultivar had the third grade on all levels. From that we can suggest Ocellatus cultivar then Albus cultivar for tissue culture work as a starting material as they had the highest content.