This work has been undertaken for the screening for Antagonist bacteria from quarry sand collected from quarry industry from dust yard of various regions of Nallambakkam, Chennai and used to screen for antagonist bacteria by using Macconkey agar. Biological control is a component of an integrated pest management strategy. It is defined as the reduction of pest populations by natural enemies and typically involves an active human role. All insect species are also suppressed by naturally occurring organisms and environmental factors, with no human input. This is frequently referred to as natural control. This guide emphasizes the biological control of insects but biological control of weeds and plant diseases is also included. Natural enemies of insect pests, also known as biological control agents, include predators, parasitoids, and pathogens. Biological control of weeds includes insects and pathogens. Biological control agents of plant diseases are most often referred to as antagonists. Identified Pseudomonas aeruginosa from quarry sand, which help to kill and control the pesticides.