High-performance liquid chromatography and UV spectrophotometric method were developed and validated for the quantitative determination of the bulk sertaconazole nitrate and its micro emulsion formulation. For HPLC, LC GC Qualisil BDS C18 column (4.6×250 mm, 5μm particle size) with the mobile phase consisted of acetonitrile-water (65:35%v/v) and flow rate of 1.8 ml/min were used for the analysis. The sertaconazole nitrate peak is monitored at a wavelength of 260 nm; the retention time was 20.16 min. The method is considered reliable for the determination of sertaconazole nitrate. Nearly 99.6% of sertaconazole nitrate from microemulsion formulation were recovered by applying this method with RSD 0.18% (n=9). UV spectrophotometric method was also found suitable for the estimation of sertaconazole nitrate with wavelength of 260 nm, linearity range 5-25 μg and correlation coefficient 0.9993, 99.25% of sertaconazole nitrate were recovered by this process with RSD 0.8%. The determination of sertaconazole nitrate was not affected by any of the vehicle used in the formulation of sertaconazole nitrate micro emulsion