Take a copper ore in Yunnan province as the object. Based on the analysis of the properties and copper phase, recovering copper concentrate from copper ore by flotation was determined. The effects of grinding fineness, collector dosage and inhibitor dosage were researched. The copper concentrate was obtained by adopting flotation process of one roughing three times concentrate and three times scavenging with the grade 20.45% and the recovery rate 86.73%. It laid a solid foundation for the further development of this copper ore.