Rational spatial structure is of great importance for the sound development of regional tourism. Anhui Province is rich in tourism resources with rapid tourism development. However, tourism development in different regions is out of balance with huge difference in inter-regional tourism. In addition, the space structure of tourism is extremely irrational. Targeting 16 cities of Anhui province as the research unit, this paper has measured the space structure of regional tourism in Anhui Province applying variable coefficient, Gini coefficient, connectivity, accessibility, regional spatial pattern, connection system of urban space, clustering analysis and other relevant methods. And, the measuring and analysis results have been dissected and interpreted in the paper. Research results indicate that the development conditions for tourism space of Anhui Province are out of balance with huge difference in developmental level and irrational spatial structure. In the meantime, the integrated development measures including enhancing the competitiveness of three major core tourism cities (Hefei, Huangshan and Wuhu), improving the regional driving and radiating capacities as well as establishing and perfecting the four major tourism areas have been proposed so as to promote coordinated development of regional tourism and provide certain theoretical and practical guidance for developing Anhui Province as a “Powerful province in tourism economy” during the 12th Five-Year Plan.