Based on the analysis of Multi-element and phase, flotation was used to enrich gold from goldmine. The effect of grinding fineness, inhibitors, activators and collectors were researched on enriching gold from goldmine by flotation. At the condition of grinding fineness -0.074mm85%, the dosage of Na2CO3 800g/t, water glass 600g/t, Cu2SO4 60g/t, butyl xanthate 400+100g/t, pine oil 60+30g/t, a fine quality gold concentrate was obtained with the grade of 31.01g/t, comprehensive gold recovery of 87.36%.