The objective of the present research is studying the relationship between body image and difficulty in emotion regulation and sexual satisfaction of healthy women with women undergoing mastectomy. Fifty afflicted women to mastectomy who were operated and fifty healthy women that were their companion was selected by convenience sampling method, the objective of this study for the members of these 2 groups was comparing body image concerns, difficulty in emotion regulation, and sexual satisfaction between healthy and mastectomy women in Isfahan in 2016. The methodology was causal-comparative. People filled difficulty in emotion regulation scale (Gratz and Roemer, 2004) with 0.86 reliability, the body image concern inventory (Littleton, 2005) with 0.89 reliability, and Larson sexual satisfaction questionnaire (Larson et al., 1998) with 0.93 reliability. SPSS 22 software, descriptive statistical method (mean, standard deviation), and inferential statistical method (multivariate variance analysis) were used to analyze the hypotheses. Results showed that there is not significant difference between difficulty in emotion regulation and its dimensions (emotional rejection, difficulty in carrying out purposeful behavior, impulse control difficulty, lack of emotional awareness, limited access to emotion regulation strategies, lack of emotional clarity, and total difficulty) in mastectomy and healthy women. (P>0.05). There is not significant difference between body image concern in mastectomy and healthy women. (P>0.05). In addition, results showed that there is significant difference between sexual satisfaction in mastectomy and healthy women. (P<0.05)