Essential oil from rose petals roses are very small, the average ranged from 0.01 to 10.0%. Local Americana beauty red roses and roses Holand from Cipanas contains oil rose about 0.08 to 0.14%, with citronellol 27.23 % and geraniol 16.18%, in East Java (Batu) content of local Rosa hybrida varieties Hybride Tea purple containing β- citronellol 21.07 % and Geraniol 0.18 % In Indonesia, produce rose essential oil is low, compared rose essential oil of Kashmir and Bulgarian. The essential rose oil research try to improve the callus of rose leaf with elicitation abiotic. This Research of essential oil in rose leaf callus induced by elicitor Long exposures (0 hours / day, 12 hours / day and 24 hours / day) and light intensity (1200 Lux and 2400 Lux). Formation of the results obtained Geraniol need light. The content is highest Citronellol in the dark treatment