Quantification of microbial biomass is very important for proper understanding of the microbial process in environment. There are many different methods available to estimate the bacterial biomass but they all suffer from some limitations. Due to the recent development in molecular methods, it is possible to quantify the bacterial biomass by analyzing the genomic DNA. The aim of this research work was to estimate the bacterial biomass by quantification of DNA extracted from halophilic bacterial isolates during the growth. For this purpose, two bacterial strains JSO1 and JSO2 isolated from halophylic environment were used. The strains isolated were sequenced and identified as Micrococcus lylae (JSO1) and Micrococcus luteus (JSO2). After monitoring the growth, the cultured samples were used for extraction of DNA and were analyzed by UV spectrophotometer. JSO1 showed 13μg/ml growth compared to JSO2 which had 7.2μg/ml of DNA. JSO2 showed better purity of the DNA ranging from 1.72 to 1.84 than JSO1 which had 1.69 to 2.15. JSO1 showed higher DNA growth than JSO2 but purity was seen higher in JSO2 than JSO1. Relationship between turbidity and DNA concentration was significant, but the level of significance was low suggesting DNA quantification may be useful to quantify the bacterial biomass