In present study improvement against biodegradation of the jute fibers due to microbes in moist environment investigated by treatment of jute fabric with N-N-dimethylamine N-ethylamine chitosan. The effectiveness assessed by employing moist soil burial test which gives favorable environment for microbial attack and strip test which confirms the effects of chemical treatment, changes in structural details. The jute fibers on treatment showed excellent potential against microbes and indicates positive performance on strip test.