The aim of this study is to observe the hematological alterations in berberine chloride (BC) treated streptozotocin (STZ) induced diabetic rats. Diabetes was experimentally induced in healthy male rats by a single dose of intraperitonial injection of STZ (40 mg/kg b.w). After the 72 hours, diabetic rats treated with BC at different concentrations (25, 50 and 100 mg/kg b.w) for 45 days. BC administration significantly declined the level of blood glucose, white blood cells (WBC) and glycated hemoglobin (HbAlc) whereas elevated the levels of plasma insulin, hemoglobin, red blood cells (RBC), haematocrit (Ht), mean cell hemoglobin (MCH) and mean cell hemoglobin concentration (MCHC) in diabetic rats. 50 and 100 mg/kg b.w of BC showed the prominent effect compared to 25 mg/kg b.w. In which, there is no any significant between dosage 50 and 100 mg/kg b.w. These findings clearly showed that the treatment of BC notably protected the blood components against STZ induced diabetic rats.