Research on production of asiaticoside from hairy root of pegagan, Centellaasiatica (L.), was conducted using chitosan, chito-oligosaccharide (COS), carboksimethyl chitosan (CMC) as elisitors. The objectives of this research were studying the effect of addition of chitosan and its derivates with several concentrations on wet weight of hairy roots of C.asiatica and on asiaticoside content in hairy roots of C.asiatica. The research was conducted using Randomized Design (Nested Design) with 3 replications. Factor A was kind of elicitors, Chitosan, Chitooligosaccharide (COS), and Carboksimethyl chitosan. Factor B was concentrations of elisitors control, 15, 30, and 45 ppm. The results showed that addition of 30 ppm chito-oligosaccharide resulted in the highest frest weight of hairy roots of C.asiatica (551,68 mg/g) and the highest content of asiaticocide (5,97 mg/g freshweight or 5-folds of control).