There is worldwide concern regarding the development of biodegradable polymer as a remedy towards harmful effects caused by synthetic polymer. These materials which are synthesised by petrochemical process, contribute towards air pollution and waste management problem. Biodegradable polymers are able to significantly reduce the environment impact in terms of energy consumption and greenhouse effect. Biopolymers are synthesised by microbial sources and this study aims to produce biopolymer from Pseudomonas fluorescens (pf01) by fermentation process. Biopolymers produced by bacterial species were screened by Sudan Black B staining method. The polymer extraction was carried out by Sodium Hypochlorite method. The maximum biopolymer production of 55% was observed at 72 hrs. FTIR and DSC methods were carried out for the characterisation process.