The objective of the present study was to investigate the presence of phytochemicals constitution and some biochemical parameters of ethanolic leaves extract of Mentha spicata plant. Soxhlet apparatus was used for the organic solvent extraction. Solvents used were ethanol-water (80:20) respectively. Qualitative analysis of phytochemical constituents of (alkaloids, phenols, glycosides, tannins, saponins, flavonoids, steroids and terpenoids) and some biochemical parameters of ethanolic extract by measuring (Blood Sugar, Blood Urea, Serum Creatinine, Cholesterol, Triglycerides, Total Serum Bilirubin). The results shown the phytochemical screening revealed the extract richness in alkaloids, tannins, saponins, flavonoids, phenols, glycosides and steroids. While the terpenoids not detectable. The results of blood sugar and serum creatinine showed significantly increased within (T2) through exposure to sodium nitrite at a level (P<0.01), while (T3) show significantly increased at a level (P<0.05), but (T4) did not showed any significant difference. Blood urea show significantly increased within (T2) at a level of (P<0.01), while (T3, T4) did not showed any significant difference. Cholesterol results showed significantly increased within (T2) at a level of (P<0.01), while (T3, T4) showed significant difference at a level (P<0.05). Triglycerides showed significantly increased within (T2, T4) at a level of (P<0.01), while (T3) did not showed any significant difference. Serum bilirubin did not showed any significant difference of (T2, T3 and T4) in comparison with (T1). To conclude, ethanolic extract of Mentha spicata contains; alkaloids, phenol, steroid, flavonoids, saponins, glycosides and tannins.