Tiny satellites or simple sequences are of DNA molecular markers and has the repeating units of2-6 pair matches. Maintaining genetic diversity in order to modify crops and to improve the levels of genetic resources management has a great importance in maize breeding programs. Also, they have a great importance in forensic identification, genomic mapping and analysis of genetic linkage. It was estimated that around 400 millions of STR markers are exist in the genome which most of them have polymorphic by different. Various lengths in alleles are caused by the differences in the number of times that the original monomer sequence has been repeated. Since the STRs are easily reproducible by PCR, PCR analyzing methods with the help of STR or STR Typing is very useful technique in the field of genomic DNA profiling. This method is more sensitive than alignment techniques such as RFLP. Because of such benefits, these markers are used as selective markers in forensic identification, particularly in destructed cases. 100 blood samples from unrelated-blood-healthy volunteers that three generations of them have been living in the Kurdish regions of Lorestan, i.e. their race were of Kurds, were collected for the present study. Salting out method was then used in order to extract DNA. Monoplex PCR was carried out for each locus of (D3S1358, D16S539) and finally, detection and typing of alleles have been done by agarose gel electrophoresis 5%. The obtained data were analyzed by statistical software of Genepop. Both locus of (D3S1358, D16S539) have polymorphism and heterozygosity which were more than 0.7; were the most polymorphisms (0.7805/0) and heterozygosity (0.8500) related to the locus of D16S539. The applicable results of these two locus have been approved in population identification survey, respectively. In addition, D16S539 Locus showed a more degree of heterozygosity and polymorphism rather than D3S1358 in the population of Lorestan.