The present study was designed to investigate the effects of seed pre-sowing treatment with 24-epibrassinolide (EBL) and Salicylic Acid (SA) combination on oxidative damage, elemental analysis, osmolyte content and metal chelating compounds in 90 days old Brassica juncea L. plants under lead (Pb) metal stress. The results showed that increasing Pb concentration increased oxidative damage, osmolyte content and metal chelating compounds whereas the contents of elements were observed to be reduced in response to Pb metal treatment. Combined treatment with EBL and SA led to further enhancement in contents of osmolytes and metal chelating compounds.