Medicinal plants and its phytoconstituents were estimated the presence of various secondary metabolites including flavonoid, phenol, tannin, flavonoid, saponins, steroids, cardiac glycosides steroids and terpenoids in the leaf ethanolic extracts of Vitex negundo and Calatropis gigantea. And the quantitative estimation of total phenolic content was estimated to be 261 and 181 mg gram equivalents of catechol of Vitex negundo and Calatropis gigantea respectively. The total flavonoid content was expressed in Quercetin gram equivalents of 278 and 239 mg equivalents per gram of the extract of Vitex negundo and Calatropis gigantea. The study reveals the phytoconstituents presence in the leaf extracts which showed potential activity and it can be developed in active ingredient for the development of pharma molecule.