Kulathadi kasahaym is an ayurvedic formulation used for treatment of Amenorrhoea. The main ingredients of this medicine seeds of horse gram, sesame, errand and garlic. This is a liquid formulation. GC MS analysis of this medicine is done in the present study to know the various bioactive compounds present therein. It was found that major peaks represented Benzoic acid, Diethyl Phthalate, Tetratricontane, Octadecanoic acid, n-Hexadecanoic acid, Heptacosane 1-Chloro, Eicosane, Phenol, 2,4-bis(1,1-dimethylethyl)-, Eicosane, 2-methyl-, Nonadecane, 2- methyl-, Hexadecane 2,6,11,15- tetramethyl-, Heptadecane, 2,6,10,15-tetramethyl- and Eicosane, 10-methylderivative. The medicinal values of most of these compounds indicate that this medicine does have a scientific indication as an effective cure for Ammenorrhorea. Further work is in progress to prove the validity of this medicine