In the present study investigate effect of different fractions isolated from the methanolic extract of whole plant of Hygrophila auriculata on cataract induced by glucose. Goat eye lens were divided into five groups. Group I lens were incubated in artificial aqueous humor with glucose concentration 5.5 mM (normal control). Group II lens were incubated with glucose concentration 55 mM (toxic control). Group III, IV and V lens incubated with glucose concentration 55 mM were incubated along with fraction A (4mg/ml), fraction B (4mg/ml) and fraction F (4mg/ml) and subjected to photographic evaluation for opacity. The grades of opacity was 0, +++, +, ++ and +++in group I, II, III, IV and V, respectively. Fraction A showed potent prevention on in vitro glucose induced cataract. Thus, the goat lens model could be used for testing of various anticataract agents.