The prasugrel characterization of reference substance described in this work ensures its purity and identity. The melting point by DSC (122.03 °C) and capillary method (120.5- 121.9 °C) demonstrated consistent results in both methods. In addition, the IR spectrum, ¹H NMR and 13C NMR spectroscopy results are presented and discussed in this study. For qualitative analysis of pharmaceutical, Rf (0.64) results obtained by TLC, chromatographic (6.02 minutes) and spectroscopic methods verified the identity of the samples. The methods employed in this paper were suitable for the identification and qualitative quantification of prasugrel and provides important information for quality and the conformity of products containing this drug. The information available emphasized the important role the qualification, especially, of the secondary reference standards.