The aim of this study is to Evaluate the anti-diabetic effect of otolith (ear stone ) that obtained from type of fish called "Arius Thalassinus ", which has medicinal properties and traditionally used in Yemen", for treatment of diabetic mellitus type 2. Experimental animals were divided to 2 diabetic models (streptozotocin and fructose model) Composed of ( 9) groups , Each model contains 4 groups , 6 animals in each group , while, Group I was kept as control for both models only taken distilled water (2ml/kg) . Administration of otolith in the doses of 2g and 3g/kg of body weight in STZ model showed significant (P<0.05) reduction in blood glucose levels, cholesterol ,TG, and LDL compared to diabetic control rats, with no effect on body weight . On the other hand, administration of otolith in the doses of 2g and 3g/kg in fructose model showed significant (P<0.05) reduction in blood glucose, insulin, cholesterol, TG, LDL levels respectively as well as body weight compared to diabetic control rats. In addition, otolith 2 and3g/kg showed high reduction in cholesterol level than reference drug pioglitazone, in fructose model. Administration of otolith 3g/kg produced significant higher anti diabetic activity than otolith 2g/kg dose. In conclusion, The traditional use of otolith to treat diabetes is supported by laboratory Findings from this study.