Musa acuminata Colla (AA) ‘Karivazhai’, an interesting hill banana is endemic to Kolli hills of Tamil Nadu and is cultivated for its sacred uses there. The plant is characterized by the black pseudostem and fruits with an excellent flavour and taste. The present study was undertaken to explore the potential bioactive fatty acids and volatile compounds present in the unripe fruit pulp of this critically endangered banana cultivar using Gas Chromatography–Mass Spectrometry analysis. The analysis revealed the existence of 15 important compounds, which will be further considered for pharmacological activities and isolation of individual components would however, help to find new drugs. This also shows the importance as a medicinal plant and the need of conserving it.