Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2013 Vol: 5 Issue: 4

Phytochemical screening and free radical scavenging activities of orange baby carrot and carrot (Daucus carota Linn.) root crude extracts

Abstract

Daucus carota Linn., commonly known as carrot, is a vegetable which has beta-carotene content. Roots of Daucus carota Linn.have been used for treatments of inflammation and some chronic diseases. However, active compounds in carrot roots that makes these treatments effective remain unknown. The major focus of this study was to determinetotal phenolic, total flavonoid contents and antioxidant activities in petroleum and ethanolic extracts from baby carrot and carrot. Baby carrot and carrot root crude extracts were assayed for total phenolic and total flavonoids contents, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2’-Azino-bis(3- ethylbenzthiazoline-6-sulphonic acid) (ABTS) and radical scavenging activity. Ethanolic extracts from baby carrots and carrots showed total phenolic content of 35.9±4.0 and 30.7±3.1 mg GAE/g dry plant material respectively. No phenolic contents were detected in petroleum extracts. Total flavonoid content of ethanolic extracts from baby carrot and carrot were 35.3±6.8 and 20.4±2.8 mg QE/g dry plant material, respectively. Petroleum extracts of baby carrot and carrot had total flavonoid contents of 17.7±2.7 and 3.7±0.7 mg QE/g dry plant material, respectively. Interestingly, ethanolic extracts of baby carrot and carrot had higher antioxidant activities as compared to those of petroleum extracts as measured by DPPH and ABTS assay. Thesefindings could be further used in future investigations which may lead to a development of new drugs for prevention or treatment of chronic diseases that involve damages caused by free-radicals

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