Ethanolic leaves extract of Alseodaphne semecarpifolia subjected to successive extraction using the solvents like n-hexane, toluene, chloroform, ethyl acetate and methanol. Prepared various solvents leaves extracts were then subjected to preliminary phytochemical and biochemical studies. It was found that the leaves extract contains steroids, alkaloids, phenolics, flavonoids, glycosides, essential oils, tannins and saponins etc. The ethanolic extract was selected for the antimicrobial activity against different pathogens of 20 bacteria and 6 fungus and it was determined by using Agar Well diffusion method. Moreover, this extract showed inhibitory effects due to the above mentioned phytochemicals present in the leaves.