The exhaustive extraction of Securidaca longepedunculata (Fresen) root bark harvested few days after rainy season with methanol gave an extract yield (19.82 %), which was then exhaustively fractionated with solvents of increasing polarity namely- n-hexane, chloroform, and ethyl acetate. The remaining residue after solvent fractionation was regarded as methanol fraction. These four fractions were analyzed for their antimicrobial activity against some multidrug resistant pathogenic microorganism and phytochemical screening was carried out to determine the secondary metabolites in each fraction. The antimicrobial analysis revealed that the chloroform fraction was the most active with mean zone of inhibition (23 mm) followed by methanol fraction (21 mm), ethyl acetate fraction (19 mm) and hexane fraction (15.5 mm). The chloroform fraction was highly potent against methicillin resistant- Staphylococcus aureus (MRSA), Streptococcus pyogenes, vancomycin-resistant Klebsiella pneumonia and multidrug resistant Pseudomonas aeruginosa and Pseudomonas fluorescens. Phytochemical screening revealed that cardiac glycosides, flavonoids, tannins, and saponins are present in the entire fractions, alkaloids were present in methanol and ethyl acetate fractions only, tannins are present in all the fractions except hexane fraction and anthraquinones were absent in the entire fractions. The presence of these secondary metabolites accounts for the antimicrobial activities of the fractions obtained from the root bark extract of this plant and also, justified the claimed traditional medicine use in the treatment of bacterial infections.