Preliminary studies on the phytochemistry and extract of diethyl ether, chloroform and acetone of Cassia alata L leaf, flower and seed coat were examined for antimicrobial properties. Extract tested against clinical isolated of Pseudomonas sps, Escherichia coli, Staphylococcus sps, Shigella boydii and Salmonella sps. The zone of inhibitions produced by the extracts in disc diffusion assay against the test microorganisms, the produced zones that measured in mm. Preliminary phytochemical analysis showed that the extracts contained tannin, flavonoid, terpenoid, cardiac glycosides, steroids and terpenoids, absence of alkaloids.