Red chilli is a commercial crop which is used in a wide range of food products in India as well as across the world. The varieties of red chilli available in different geographical zones of India show remarkable variation in their pungency. There is absolutely no information available on its standardization and quality control which of course is essential for establishing authenticity, quality and efficacy of herbal products. In the present study an attempt has been made to standardize red chilli samples collected from different geographical locations. The method developed for standardization employed qualitative evaluation of physicochemical characters and HPTLC profiles.