After carrying out an ethnomedical survey which has showed an intensive use of Commiphora africana as traditional healing medicinal plant in Mauritania [1], we chose to investigate the oleoresin as the mostly used by Mauritanian healers. Oleoresin from identified plant has been collected in from 4 Mauritanian localities and extracted with the aid Soxhlet apparatus; and cyclohexane to give extracts MC, KC, RC and GC. Colorimetric test has suggested the presence of flavonoids and terpenoids in these extracts. In addition essential oils in oleoresin have been analyzed by modern techniques like Head Space, Clevenger and hydrodistillation. Furthermore cyclohexane extracts have been subjected to fractioning on a Si-Ge column and resulting fractions (MCF, KCF, RCF and GCF) were tested against Escherichia coli and Staphylococcus aureus strains and, in parallel, analyzed by HPLC_UVDAD and RP-UHPLC_ESItof. This bioguided profiling has permitted to establish for a first time a profiling of flavonoids by RP_HPLC_UVDAD in this plant part and also to present ad replication by RPUHPLC_ ESItof of bioactive compounds, like terpenoids, already identified in other commiphora oleoresins.