The phytochemical studies on the plant of Mimosa pudica resulted in isolation of Mimosine ( 5 α amino 3 hydroxy 4 oxo 1 H (H) pyridine propianic acid), α- spinasterol, Phenyl ethylamine derivatives are being reported for the first time from this plants. These compounds have been characterized on the basis of spectral and other data. The isolated compounds (JS I, JS II, JS III and JS IV ) were found out in amylase and urease enzyme. The isolated compounds JS IV only showed high enzyme inhibitory activity.